3CSL
Structure of the Serratia marcescens hemophore receptor HasR in complex with its hemophore HasA and heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 157.020, 163.400, 595.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.174 - 2.700 |
| R-factor | 0.2406 |
| Rwork | 0.239 |
| R-free | 0.27390 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.535 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.174 | 2.710 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.164 | 0.722 |
| Number of reflections | 111662 | |
| <I/σ(I)> | 10.21 | 1.59 |
| Completeness [%] | 91.0 | 63.5 |
| Redundancy | 6.03 | 2.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 2 M NaCl, 100 mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 2 M NaCl, 100 mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
