3CSG
Crystal Structure of Monobody YS1(MBP-74)/Maltose Binding Protein Fusion Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-26 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 |
Unit cell lengths | 68.578, 68.578, 108.002 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.798 |
R-factor | 0.19219 |
Rwork | 0.187 |
R-free | 0.23530 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.911 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.798 | 1.798 |
Number of reflections | 46107 | |
Completeness [%] | 99.7 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 292 | 20% polyethyleneglycol-1000, 0.1 M Na/K phosphate, 0.2 M NaCl, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |