3CS5
NblA protein from Synechococcus elongatus PCC 7942
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 4 |
| Unit cell lengths | 78.080, 78.080, 70.678 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| Rwork | 0.248 |
| R-free | 0.34660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q8v |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 21001 |
| <I/σ(I)> | 9.75 |
| Completeness [%] | 96.9 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 25% ethylene glycol, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
