3CS1
Flagellar Calcium-binding Protein (FCaBP) from T. cruzi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.910, 37.670, 141.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.212 |
| R-free | 0.28800 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.741 |
| Data scaling software | d*TREK (9.1SSI) |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.840 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.308 | |
| Number of reflections | 12558 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.8 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | well solution: 2.88M ammonium sulfate, 0.09M MES pH 6.0, 0.075 magnesium chloride, 0.01M sodium acetate pH 4.6., hanging drop vapor diffusion, temperature 298K, pH 6.00 |
