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3CRX

CRE RECOMBINASE/DNA COMPLEX INTERMEDIATE I

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date1997-05-15
DetectorMARRESEARCH
Spacegroup nameC 2 2 21
Unit cell lengths106.300, 122.700, 179.500
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution27.300 - 2.500
R-factor0.198
Rwork0.198
R-free0.26800
Structure solution methodDIFFERENCE FOURIER
Starting model (for MR)1crx
RMSD bond length0.007
RMSD bond angle23.100

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareX-PLOR
Refinement softwareX-PLOR (3.851)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.0002.780
High resolution limit [Å]2.7002.700
Rmerge0.072

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0.329

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Number of reflections38807
<I/σ(I)>205
Completeness [%]99.093.2
Redundancy4.74.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

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5.7

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4

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50MM ACETATE PH 5.0, 26% MPD, 80MM MGCL2
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropCre0.065 (M)
101reservoirMPD24 (%)
111reservoir20 (mM)
121reservoir200 (mM)
21droploxS6 half-site0.081 (M)
31dropsodium acetate25 (mM)
41dropMPD12.5 (%)
51drop5 (mM)
61drop120 (mM)
71dropdithiothreitol2.5 (mM)
81dropspermine0.5 (mM)
91reservoirsodium acetate50 (mM)

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PDB entries from 2024-10-30

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