3CRW
XPD_APO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11583 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.383, 69.805, 145.144 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.000 - 4.000 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.31900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3crv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 4.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 3.110 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Number of reflections | 9404 | |
| Completeness [%] | 81.5 | 37.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 0.4M Citrate pH 5.6 15-20% PEG 4000 10% Isopropanol, VAPOR DIFFUSION, temperature 298K |
