3CRQ

Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-15
Wavelength(s)	0.98
Spacegroup name	P 41
Unit cell lengths	76.312, 76.312, 57.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.220
R-free	0.25000
RMSD bond length	0.006
RMSD bond angle	1.150
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	16614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	278	PEG 6000, pH 8.5, vapor diffusion, hanging drop, temperature 278K