3CRC
Crystal Structure of Escherichia coli MazG, the Regulator of Nutritional Stress Response
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.23985 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.230, 66.910, 140.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.790 - 3.000 |
| R-factor | 0.22233 |
| Rwork | 0.216 |
| R-free | 0.34510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.041 |
| RMSD bond angle | 3.742 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 12574 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1M trisodium citrate(pH 5.5), 0.2M ammonium acetate, 20% polyethylene glycol 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
