3CR7
Crystal structure of N-terminal truncation of APS Kinase from Penicillium chrysogenum: Ternary structure with ADP and PAPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9194 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.580, 82.594, 139.009 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.715 - 2.500 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | poly alanine model of pdb entry 1M7G A chain |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.421 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | EPMR |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.718 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.084 | 0.486 |
| Total number of observations | 8337 | |
| Number of reflections | 31427 | |
| <I/σ(I)> | 7.5 | 1.5 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | bis tris, PEG 3350, ammonium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
