3CR3
Structure of a transient complex between Dha-kinase subunits DhaM and DhaL from Lactococcus lactis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97885,0.97823,0.97240 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.133, 146.842, 58.483 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.230 - 2.100 |
| R-factor | 0.22168 |
| Rwork | 0.219 |
| R-free | 0.26300 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.215 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 147.442 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.047 | 0.161 |
| Number of reflections | 37982 | |
| <I/σ(I)> | 22.2 | 8.1 |
| Completeness [%] | 99.8 | 24.4 |
| Redundancy | 4.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1 M Tris/HCl, 0.2 M LiSO4, 36 % PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
