3CR1
crystal structure of a minimal, mutant, all-RNA hairpin ribozyme (A38C, A-1OMA) grown from MgCl2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-06-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.977 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.640, 93.640, 133.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.890 - 2.250 |
Rwork | 0.219 |
R-free | 0.24600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2oue |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.890 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.040 | 0.541 |
Number of reflections | 16729 | |
<I/σ(I)> | 25.8 | 4.4 |
Completeness [%] | 98.2 | 99.7 |
Redundancy | 11.3 | 11.01 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG2000 MME, lithium sulfate, magnesium chloride, spermidine, sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG2000 MME | ||
10 | 1 | 2 | sodium cacodylate | ||
2 | 1 | 1 | lithium sulfate | ||
3 | 1 | 1 | magnesium chloride | ||
4 | 1 | 1 | spermidine | ||
5 | 1 | 1 | sodium cacodylate | ||
6 | 1 | 2 | PEG2000 MME | ||
7 | 1 | 2 | lithium sulfate | ||
8 | 1 | 2 | magnesium chloride | ||
9 | 1 | 2 | spermidine |