3CQ9
Crystal structure of the lp_1622 protein from Lactobacillus plantarum. Northeast Structural Genomics Consortium target LpR114
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97885, 0.97938, 0.96785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.784, 194.031, 52.093 |
Unit cell angles | 90.00, 115.34, 90.00 |
Refinement procedure
Resolution | 44.670 - 2.200 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.20400 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXDE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.050 | 0.076 |
Number of reflections | 88438 | |
<I/σ(I)> | 32.9 | 19.3 |
Completeness [%] | 95.1 | 79.7 |
Redundancy | 3.4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 18% PEG 3350, 0.2M Ammonium sulfate, 0.1M Sodium acetate pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |