3CQ4
Histidinol-phosphate aminotransferase from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 102.340, 102.340, 140.108 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.23908 |
| Rwork | 0.237 |
| R-free | 0.27674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uu1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.347 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.900 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.051 | 0.530 |
| Number of reflections | 43528 | |
| <I/σ(I)> | 25.5 | 4.7 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 9% PEG 6K, 1M lithium acetate, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
