3CPX

Crystal structure of putative M42 glutamyl aminopeptidase (YP_676701.1) from Cytophaga hutchinsonii ATCC 33406 at 2.39 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97859
Spacegroup name	P 61 2 2
Unit cell lengths	83.840, 83.840, 682.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.656 - 2.390
R-factor	0.189
Rwork	0.187
R-free	0.22300
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.818
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.656	29.656	2.600
High resolution limit [Å]	2.390	10.000	2.390
Rmerge	0.055	0.029	0.616
Number of reflections	52024	1389	16895
<I/σ(I)>	16.87	40.9	2.27
Completeness [%]	88.6	94.9	73.5
Redundancy	7.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	NANODROP, 1.0M LiCl, 10.0% PEG 6000, 0.1M MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K