3CPQ

Crystal Structure of L30e a ribosomal protein from Methanocaldococcus jannaschii DSM2661 (MJ1044)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-06-13
Detector	RIGAKU
Wavelength(s)	1.0
Spacegroup name	P 43
Unit cell lengths	46.115, 46.115, 98.509
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.120 - 1.900
R-factor	0.202
Rwork	0.202
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w41
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.067	0.478
Number of reflections	16282
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	291	40% PEG400, 0.1 MES, 5% PEG3000, pH 6.0, MICROBATCH, temperature 291K