3CPI
Crystal structure of yeast Rab-GDI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.460, 159.780, 77.093 |
| Unit cell angles | 90.00, 90.25, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.300 |
| R-factor | 0.1992 |
| Rwork | 0.197 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ukv chain G |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 59630 | |
| Completeness [%] | 98.5 | 80 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
