3CP7
Crystal structure of a thermostable serine protease AL20 from extremophilic microoganism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.076 |
| Spacegroup name | H 3 |
| Unit cell lengths | 92.260, 92.260, 137.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.180 - 1.390 |
| R-factor | 0.17887 |
| Rwork | 0.178 |
| R-free | 0.19904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p3e |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.314 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.440 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.049 | 0.193 |
| Number of reflections | 86325 | |
| <I/σ(I)> | 14.5 | 3.5 |
| Completeness [%] | 98.0 | 89.4 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 294 | 2.9M sodium formate, 0.1M sodium citrate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
