3CP5
Cytochrome c from rhodothermus marinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-24 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.592, 32.644, 42.064 |
| Unit cell angles | 90.00, 94.56, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.240 |
| R-factor | 0.1299 |
| R-free | 0.17440 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.032 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.410 | 1.300 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.084 | 0.480 |
| Number of reflections | 29745 | |
| <I/σ(I)> | 13.6 | 1.6 |
| Completeness [%] | 96.3 | 75.9 |
| Redundancy | 9.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 2.2 | 293 | 30% PEG8K, 0.2M ammonium sulfate, 8% hexanediol, 50mM sodium citrate, pH2.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
