3COQ
Structural Basis for Dimerization in DNA Recognition by Gal4
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 298 |
| Wavelength(s) | 1.2823,1.2830,1.2448 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.495, 40.829, 90.418 |
| Unit cell angles | 90.00, 95.88, 90.00 |
Refinement procedure
| Resolution | 28.940 - 2.400 |
| R-factor | 0.213 |
| Rwork | 0.207 |
| R-free | 0.26900 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 3.337 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.066 | 0.417 |
| Number of reflections | 18311 | |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 98.1 | 97 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | 40mM Mg(OAc)2, 25mM sodium phosphate, 5% PEG400, 5% MPD, pH 5.5, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | 40mM Mg(OAc)2, 25mM sodium phosphate, 5% PEG400, 5% MPD, pH 5.5, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Mg(OAc)2 | ||
| 2 | 1 | 1 | sodium phosphate | ||
| 3 | 1 | 1 | PEG400 | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | Mg(OAc)2 | ||
| 6 | 1 | 2 | sodium phosphate | ||
| 7 | 1 | 2 | PEG400 | ||
| 8 | 1 | 2 | MPD |
