3COI
Crystal structure of p38delta kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.271, 71.305, 97.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.090 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oza |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.263 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.200 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.120 | 0.627 |
| Number of reflections | 27415 | |
| <I/σ(I)> | 10.6 | 4.6 |
| Completeness [%] | 97.3 | 82.6 |
| Redundancy | 5.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 10% Glycerol, 20mM Hepes pH 7.5, 150mM Sodium chloride, 10mM Methionine, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
