3COD
Crystal Structure of T90A/D115A mutant of Bacteriorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.974, 109.658, 55.517 |
| Unit cell angles | 90.00, 113.37, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.700 |
| Rwork | 0.273 |
| R-free | 0.28700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
| Rmerge | 0.134 | 0.070 | 0.219 |
| Number of reflections | 12143 | ||
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 91.0 | 99.1 | 65.1 |
| Redundancy | 2.8 | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.7 | 310 | 2.4 M NaH2PO4 (pH 3.7), 3.5% triethylene glycerol, and 0.15 M hexanediol, bicelle method, temperature 310K |
