3COC
Crystal Structure of D115A mutant of Bacteriorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.362, 108.966, 56.432 |
| Unit cell angles | 90.00, 113.72, 90.00 |
Refinement procedure
| Resolution | 29.710 - 2.310 |
| Rwork | 0.221 |
| R-free | 0.27500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.130 | 0.085 | 0.365 |
| Number of reflections | 20963 | ||
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 93.8 | 99.2 | 84 |
| Redundancy | 3 | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 310 | 1.4 M NaH2PO4 (pH 3.7), 0.8 M NaH2PO4 (pH 4.5), and 0.12 M hexanediol, bicelle method, temperature 310K |
