3COB
Structural Dynamics of the Microtubule binding and regulatory elements in the Kinesin-like Calmodulin binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-11-18 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.152, 84.907, 89.661 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.200 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sdm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 37114 | |
<I/σ(I)> | 13.7 | 3.1 |
Completeness [%] | 95.5 | 84.8 |
Redundancy | 7.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9.1 | 298 | 20% PEG 3350, 0.2M di-Sodium hydrogen Phosphate, pH 9.1, temperature 298.0K |