3CO2
Mlotik1 ion channel cyclic-nucleotide binding domain mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Collection date | 2007-04-01 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 111.473, 132.349, 39.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.030 - 2.900 |
| R-factor | 0.26313 |
| Rwork | 0.261 |
| R-free | 0.30605 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.953 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.079 | 0.612 |
| Number of reflections | 19637 | |
| Completeness [%] | 97.9 | 89.6 |
| Redundancy | 4.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | ammoinium sulfate, Nacitrate, NaCl, VAPOR DIFFUSION, HANGING DROP |
