3CO1
Crystal structure of microtubule binding domain of human EB3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-11-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0090 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.188, 85.365, 32.161 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.680 - 1.400 |
| R-factor | 0.20161 |
| Rwork | 0.200 |
| R-free | 0.23355 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pa7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 25525 | |
| <I/σ(I)> | 10.85 | |
| Completeness [%] | 96.9 | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 0.04M potassium phosphate, 16% PEG 8000, 20% glycerol anhydrous, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
