3CNJ
Cholesterol oxidase from Streptomyces sp. F359W mutant (0.95A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-28 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.280, 72.870, 62.950 |
Unit cell angles | 90.00, 105.11, 90.00 |
Refinement procedure
Resolution | 46.670 - 0.950 |
Rwork | 0.140 |
R-free | 0.17500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1mxt |
RMSD bond length | 0.013 |
RMSD bond angle | 0.030 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.670 | 0.980 |
High resolution limit [Å] | 0.950 | 0.950 |
Rmerge | 0.047 | 0.480 |
Number of reflections | 270322 | |
<I/σ(I)> | 11.2 | 1.9 |
Completeness [%] | 96.3 | 67.6 |
Redundancy | 4.22 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | PEG 8000, MANGANESE SULFATE, CACODYLATE (PH 5.2), VAPOR DIFFUSION, HANGING DROP, temperature 291K |