3CNI
Crystal structure of a domain of a putative ABC type-2 transporter from Thermotoga maritima MSB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-24 |
| Detector | APS-1 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 65.598, 65.598, 141.192 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.800 - 2.300 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.802 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.713 |
| Number of reflections | 8587 | |
| <I/σ(I)> | 65.696 | 3.4 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 13.4 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 273 | 0.1M Na Hepes, 0.2M CaCl2, 28% PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
