3CMZ
TEM-1 Class-A beta-lactamase L201P mutant apo structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-04-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.11591 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.218, 59.140, 87.802 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.030 - 1.920 |
R-factor | 0.19787 |
Rwork | 0.196 |
R-free | 0.24210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jwp |
RMSD bond length | 0.013 |
RMSD bond angle | 1.473 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.061 | 0.354 |
Number of reflections | 15609 | |
<I/σ(I)> | 14 | 2.48 |
Completeness [%] | 92.0 | 83.3 |
Redundancy | 25.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 1.6M potassium phosphate, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |