3CMZ
TEM-1 Class-A beta-lactamase L201P mutant apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-04-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.11591 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.218, 59.140, 87.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.030 - 1.920 |
| R-factor | 0.19787 |
| Rwork | 0.196 |
| R-free | 0.24210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jwp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.473 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.061 | 0.354 |
| Number of reflections | 15609 | |
| <I/σ(I)> | 14 | 2.48 |
| Completeness [%] | 92.0 | 83.3 |
| Redundancy | 25.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 1.6M potassium phosphate, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
