3CMS

ENGINEERING ENZYME SUB-SITE SPECIFICITY: PREPARATION, KINETIC CHARACTERIZATION AND X-RAY ANALYSIS AT 2.0-ANGSTROMS RESOLUTION OF VAL111PHE SITE-MUTATED CALF CHYMOSIN

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	80.210, 114.560, 72.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.195
RMSD bond length	0.022
RMSD bond angle	0.050
Refinement software	RESTRAIN

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	3.000 *
Total number of observations	149939 *
Number of reflections	22098 *
Completeness [%]	96.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	phosphate	50 (mM)
3	1	reservoir		2 (M)