3CME
The Structure of CA and CCA-PHE-CAP-BIO Bound to the Large Ribosomal Subunit of Haloarcula Marismortui
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 210.786, 297.778, 572.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.820 - 2.950 |
| Rwork | 0.198 |
| R-free | 0.25500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.261 | |
| Number of reflections | 369334 | |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 292 | PEG 6000, KCL, NH4CL, MGCL2, KACETATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 10 | 1 | 2 | KACETATE | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | NH4CL | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | KACETATE | ||
| 6 | 1 | 2 | PEG 6000 | ||
| 7 | 1 | 2 | KCL | ||
| 8 | 1 | 2 | NH4CL | ||
| 9 | 1 | 2 | MGCL2 |
