3CM8
A RNA polymerase subunit structure from virus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2007-12-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795, 0.9897 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 121.942, 121.942, 134.594 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.184 - 2.899 |
R-factor | 0.226 |
Rwork | 0.224 |
R-free | 0.26100 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.899 | 2.899 |
Number of reflections | 22964 | |
Completeness [%] | 99.7 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 1M sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 1M sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |