3CM8
A RNA polymerase subunit structure from virus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-12-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795, 0.9897 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 121.942, 121.942, 134.594 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.184 - 2.899 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26100 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.899 | 2.899 |
| Number of reflections | 22964 | |
| Completeness [%] | 99.7 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 1M sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 1M sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
