3CLZ
The set and ring associated (SRA) domain of UHRF1 bound to methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.96863 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.198, 111.359, 97.403 |
| Unit cell angles | 90.00, 90.53, 90.00 |
Refinement procedure
| Resolution | 38.630 - 2.200 |
| R-factor | 0.19097 |
| Rwork | 0.189 |
| R-free | 0.23147 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bi7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.665 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 81560 | |
| <I/σ(I)> | 9.77 | 1.35 |
| Completeness [%] | 99.2 | 95 |
| Redundancy | 4.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 12 % PEG 1500, 0.1 M BIS-TRIS, 0.2 M NACL, 0.001 M TCEP, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 1500 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | BIS-TRIS | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | TCEP | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 1500 | ||
| 7 | 1 | 2 | BIS-TRIS | ||
| 8 | 1 | 2 | NACL | ||
| 9 | 1 | 2 | TCEP |
