3CLU
Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-01 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 61 |
Unit cell lengths | 116.482, 116.482, 84.281 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.000 - 1.800 |
R-factor | 0.16963 |
Rwork | 0.168 |
R-free | 0.20395 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1o97 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.541 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 58203 |
<I/σ(I)> | 9.2 |
Completeness [%] | 99.0 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | limited digest with trypsin, 2.0 M sodium phosphate, pH 6.2, VAPOR DIFFUSION |