3CLQ
Crystal structure of a conserved protein of unknown function from Enterococcus faecalis V583
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97951, 0.97965 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.159, 61.613, 164.544 |
| Unit cell angles | 90.00, 103.94, 90.00 |
Refinement procedure
| Resolution | 43.030 - 2.500 |
| R-factor | 0.18605 |
| Rwork | 0.183 |
| R-free | 0.23972 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.546 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.030 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.117 | 0.559 |
| Number of reflections | 62694 | |
| <I/σ(I)> | 22.9 | 1.88 |
| Completeness [%] | 88.8 | 54.9 |
| Redundancy | 4.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M NaCl, 0.1M Tris-HCl, 25% w/v PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
