3CLF
HIV neutralizing monoclonal antibody YZ23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.185, 63.134, 135.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.220 |
| R-free | 0.26500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3cle |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 30712 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.1 | 97.8 |
| Redundancy | 6.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 8% PEG6000, 0.1 M Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
