3CLC
Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9322 |
| Spacegroup name | P 65 |
| Unit cell lengths | 104.480, 104.480, 139.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.060 - 2.800 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.328 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 | |
| High resolution limit [Å] | 2.800 | 15.000 | 2.800 |
| Rmerge | 0.043 | 0.013 | 0.393 |
| Number of reflections | 21198 | 71 | 2103 |
| <I/σ(I)> | 33.66 | 91 | 4.8 |
| Completeness [%] | 98.5 | 49 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 50 mM MES, 40 mM MgCl2, 25% MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | MES | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | 2 | H2O |
