3CL1
M. loti cyclic-nucleotide binding domain, cyclic-GMP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Collection date | 2004-04-15 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.202, 79.594, 49.984 |
Unit cell angles | 90.00, 99.11, 90.00 |
Refinement procedure
Resolution | 30.980 - 2.400 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.309 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.326 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.081 | 0.155 |
Number of reflections | 15482 | |
<I/σ(I)> | 2 | 3 |
Completeness [%] | 87.0 | 64.9 |
Redundancy | 3.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP |