3CKQ
Crystal Structure of a Mycobacterial Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-07-14 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.0053 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 87.025, 87.025, 103.687 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.130 - 3.000 |
R-factor | 0.22506 |
Rwork | 0.224 |
R-free | 0.25353 |
Structure solution method | Rigid body refinement |
RMSD bond length | 0.006 |
RMSD bond angle | 0.923 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.130 | |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 7931 | |
<I/σ(I)> | 7 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1 M Ammonium sulphate, 0.1 M HEPES pH 7.0, 20 mM Manganese chloride, 1 mM Sodium thiosulphate, 100 mM uridine-5 -diphosphate-glucose, VAPOR DIFFUSION, HANGING DROP, temperature 293K |