3CKG
The crystal structure of OspA deletion mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97105 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.813, 117.176, 36.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.19071 |
| Rwork | 0.189 |
| R-free | 0.22604 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.497 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.104 | 0.562 |
| Number of reflections | 24821 | |
| <I/σ(I)> | 18.6 | 3.76 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 5.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.45 | 293 | 24% PEG3350, 0.1M Imidazole, 0.2M MgCl2, 16mg/ml, 30% Glycerol for cryo, pH 6.45, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
