3CJD
Crystal structure of putative TetR transcriptional regulator (YP_510936.1) from Jannaschia sp. CCS1 at 1.79 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97961 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.283, 46.044, 60.761 |
| Unit cell angles | 99.23, 100.39, 104.79 |
Refinement procedure
| Resolution | 29.801 - 1.790 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.835 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.801 | 29.801 | 1.840 |
| High resolution limit [Å] | 1.790 | 8.010 | 1.790 |
| Rmerge | 0.078 | 0.044 | 0.755 |
| Total number of observations | 1578 | 4639 | |
| Number of reflections | 32780 | ||
| <I/σ(I)> | 7.4 | 12.3 | 1 |
| Completeness [%] | 85.8 | 96.9 | 41.6 |
| Redundancy | 3.9 | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | NANODROP, 0.457M Ammonium dihydrogen phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
