3CJ8
Crystal structure of 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase from Enterococcus faecalis V583
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.209, 55.179, 140.387 |
| Unit cell angles | 90.00, 95.63, 90.00 |
Refinement procedure
| Resolution | 45.830 - 1.950 |
| R-factor | 0.20364 |
| Rwork | 0.201 |
| R-free | 0.24845 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.464 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.150 | 0.563 |
| Number of reflections | 51845 | |
| <I/σ(I)> | 13.8 | 1.63 |
| Completeness [%] | 97.7 | 83.9 |
| Redundancy | 4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 289 | 0.2M MgCl2, 0.1M HEPES, 30% PEG 400, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
