3CIU
Site-Selective Glycosylation of Cysteine-93 beta on the Surface of Bovine Hemoglobin and its Application as a Novel Oxygen Therapeutic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-10 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.604, 73.982, 129.796 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.500 - 3.500 |
| R-factor | 0.2712 |
| Rwork | 0.268 |
| R-free | 0.33213 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g0a |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.982 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.500 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.131 | 0.332 |
| Number of reflections | 7354 | |
| <I/σ(I)> | 5.3 | 2.4 |
| Redundancy | 3 | 2.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 8.25 | 293 | 25 % Polyethylene glycol, monomethyl ether 2000, 0.2 M Trimethylamine N-oxide dehydrate and 0.1 M Tris, pH 8.25, Microbatch under oil, temperature 293.0K |
