3CIR
E. coli Quinol fumarate reductase FrdA T234A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.861, 135.468, 266.024 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 266.000 - 3.650 |
| Rwork | 0.261 |
| R-free | 0.29570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b76 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.913 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 266.000 | 3.710 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Number of reflections | 32532 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 80.7 | 55.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 10% PEG 5000 MME, 0.250M magnesium acetate, 100mM citric acid, 0.001M EDTA, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
