3CIP
Complex of Dictyostelium Discoideum Actin with Gelsolin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-BM |
Synchrotron site | APS |
Beamline | 17-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9999 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 178.881, 69.172, 56.564 |
Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
Resolution | 43.730 - 1.600 |
R-factor | 0.15211 |
Rwork | 0.150 |
R-free | 0.19739 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nm1 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.326 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.071 | 0.521 |
Number of reflections | 85829 | |
<I/σ(I)> | 36.6 | 2.1 |
Completeness [%] | 96.8 | 78.4 |
Redundancy | 7.3 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, pH7.5, 1.7M Li2SO4, 2mM ATP, 1mM EDTA, 5% Glycerol, vapor diffusion, hanging drop, temperature 293K |