3CII

Structure of NKG2A/CD94 bound to HLA-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	103
Detector technology	CCD
Collection date	2006-09-01
Detector	ADSC QUANTUM 315
Spacegroup name	I 41 3 2
Unit cell lengths	345.983, 345.983, 345.983
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.880 - 4.410
Rwork	0.321
R-free	0.35529
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kpr 1b6e
RMSD bond length	0.009
RMSD bond angle	1.088
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	4.560
High resolution limit [Å]	4.410	4.400
Rmerge	0.166	0.489
Number of reflections	22619
<I/σ(I)>	6.9	1.03
Completeness [%]	100.0	100
Redundancy	34.4	26.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	1.8M ammonium sulfate, 0.3-0.7% sucrose, 100 mM Tris (8.0), VAPOR DIFFUSION, HANGING DROP, temperature 291K