3CI9

Crystal Structure of the human HSBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	35.226, 35.226, 233.266
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.530 - 1.800
Rwork	0.195
R-free	0.23400
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.05)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.036	0.029	0.190
Number of reflections	10975
<I/σ(I)>	19.1
Completeness [%]	92.1	90.9	58.9
Redundancy	3.2	3.5	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	1.7M Ammonium Sulfate,15%(v/v) Glycerol, 20mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	1.7M Ammonium Sulfate,15%(v/v) Glycerol, 20mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K