3CI0
The Crystal Structure of the GspK-GspI-GspJ complex from enterotoxigenic Escherichia coli Type 2 Secretion System
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-15 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.54704 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 96.118, 96.118, 108.436 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.960 - 2.200 |
R-factor | 0.19492 |
Rwork | 0.192 |
R-free | 0.25942 |
Structure solution method | MIRAS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.279 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.200 | 36.150 | 2.320 |
High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
Rmerge | 0.078 | 0.053 | 0.537 |
Number of reflections | 26464 | ||
<I/σ(I)> | 5.9 | 10.7 | 1.4 |
Completeness [%] | 100.0 | 98.9 | 100 |
Redundancy | 6.8 | 6.3 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 25-30% PEG 3350, 0.1M NA ACETATE (PH 5.0), 0.1M CACL2, vapor diffusion, sitting drop, temperature 298K |