3CHX
Crystal structure of Methylosinus trichosporium OB3b particulate methane monooxygenase (pMMO)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 113.810, 184.070, 203.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.000 - 3.900 |
Rwork | 0.342 |
R-free | 0.37700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yew |
RMSD bond length | 0.011 |
RMSD bond angle | 1.864 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.969 | 4.110 |
High resolution limit [Å] | 3.900 | 3.900 |
Rmerge | 0.086 | 0.376 |
Total number of observations | 43098 | |
Number of reflections | 39753 | |
<I/σ(I)> | 7.5 | 2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M cacodylate, 10% PEG3000, 0.25 M manganese chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |