3CHX
Crystal structure of Methylosinus trichosporium OB3b particulate methane monooxygenase (pMMO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.810, 184.070, 203.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.000 - 3.900 |
| Rwork | 0.342 |
| R-free | 0.37700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yew |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.864 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.969 | 4.110 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.086 | 0.376 |
| Total number of observations | 43098 | |
| Number of reflections | 39753 | |
| <I/σ(I)> | 7.5 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M cacodylate, 10% PEG3000, 0.25 M manganese chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
