3CHR
Crystal structure of leukotriene A4 hydrolase in complex with 4-amino-N-[4-(phenylmethoxy)phenyl]-butanamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.091 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.346, 133.210, 83.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.620 - 2.200 |
| R-factor | 0.20948 |
| Rwork | 0.207 |
| R-free | 0.26694 |
| Structure solution method | OTHER |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.405 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.680 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 38521 | |
| <I/σ(I)> | 6.9 | 2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 8 | 298 | PEG 8000, SODIUM ACETATE, IMIDEAZOLE PH 6.8, YBCL2, BESTATIN, LIQUID DIFFUSION, TEMPERATURE 298K, pH 8.00 |
