3CHQ
Crystal structure of leukotriene a4 hydrolase in complex with N5-[4-(phenylmethoxy)phenyl]-L-glutamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 91.6 |
| Detector technology | CCD |
| Collection date | 2003-01-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.234, 133.262, 84.591 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.830 - 2.090 |
| Rwork | 0.206 |
| R-free | 0.24000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNX |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.200 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 46299 | |
| <I/σ(I)> | 13.1 | 4.4 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 8 | 298 | PEG 8000, SODIUM ACETATE, IMIDEAZOLE PH 6.8, YBCL2, BESTATIN, LIQUID DIFFUSION, TEMPERATURE 298K,pH 8.00 |
