3CHQ

Crystal structure of leukotriene a4 hydrolase in complex with N5-[4-(phenylmethoxy)phenyl]-L-glutamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	91.6
Detector technology	CCD
Collection date	2003-01-18
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	68.234, 133.262, 84.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.830 - 2.090
Rwork	0.206
R-free	0.24000
Structure solution method	OTHER
RMSD bond length	0.005
RMSD bond angle	0.800
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNX
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.200
High resolution limit [Å]	2.090	2.090
Number of reflections	46299
<I/σ(I)>	13.1	4.4
Completeness [%]	99.7	99.8
Redundancy	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	8	298	PEG 8000, SODIUM ACETATE, IMIDEAZOLE PH 6.8, YBCL2, BESTATIN, LIQUID DIFFUSION, TEMPERATURE 298K,pH 8.00